Biotech Glossary |
Bioinformatics |
Lab Protocol |
Notes |
Malaysia University |
2. Nco I and Not I Restriction Digestion of the scFv Gene Repertoires
3. Absorption Spectroscopy and Quantification of Filamentous Phage
4. Affinity Selection of Phage
5. Amplification of a Large Phage Display Library without Losing Diversity
6. Biotinylation of Antibodies in Phage Display
7. Calcium Chloride Transfection of Bacterial Cells with Bacteriophage DNA
8. Cleavage of Bacteriophage Vector f88-4 with HindIII and PstI
9. Cleavage of Vector DNA with Restriction Enzyme(s) and Removal of "Stuffer" DNA
10. Constructing a Random-Peptide Library for Phage Display
11. General Protocol for Performing Serial Dilutions
12. Isolation of Crude Replicative Form (RF) DNA from Mid-Size Cultures of Bacteria Harboring fd-ted-based Vectors
13. Isolation of Crude RF DNA from Small Cultures of Bacteria Harboring fd-ted-based Vectors
14. Large Scale Extraction of Filamentous Bacteriophage Replicative Form (RF) DNA
15. Large Scale Purification of Wild-Type Virions Using Sarkosyl
16. Large-Scale Propagation of Frameshift Vectors (fUSE1, fUSE3 and fUSE5)
17. Large-Scale Purification of fd-tet-Derived Virions Using Sarkosyl
18. Phage Capture Assay: Micropanning
19. Phage Capture Assay: Quantifying Binding of Receptor to Phage Clones
20. Plating Plaques
21. Propagation and Processing of Phage
22. Synthesis of Degenerate Oligonucleotide Inserts for Phage-Display Libraries
23. The Smith Lab Phage Display Vectors
24. Titering Tetracycline Transducing Units
25. Transfection of Cells with Recombinant Phage DNA by Electroporation